Note:

1. Store vials containing fibers between 2-8℃ prior to use.
2. Package is sterile upon delivery.
3. For efficient resuspension of fibers, optimize concentration based on application.
4. If adding to OkaGel Solid, disperse µCollaFibR™ in 1X PBS prior to adding the required mass of OkaGel solid for desired final concentrations of µCollaFibR™ and OkaGel.
5. If adding to OkaGel liquid, disperse µCollaFibR™ directly at the desired concentration or dilute using 1X PBS.
6. For further sterilization and re-use of included metal balls, soak in 70% ethanol for 15 minutes. Wash with sterile PBS twice prior to use and dry in a Biosafety cabinet (BSC).

1. Pull apart µCollaFibR^TM into small pieces before resuspending in OkaGel (see note for use in solid vs liquid OkaGel).

2. Resuspend fibers at desired concentration (1-7.5 mg/mL tested in-house). 

For fiber concentrations below 2.5mg/mL

3. Vortex at 2500-3000 RPM for 8 min.
4. Using a magnetic stirrer, mix the µCollaFibR™ incorporated OkaGel overnight on a hot plate at 40℃ to remove bubbles and ensure even dispersion (if material needs to be sterile, do this in a BSC).
5. The bioink is now ready to use in your applications.

For fiber concentrations between 2.5mg/mL and 5mg/mL

3. Vortex with provided metal balls at 2500-3000 RPM for 6 min.
4. While bioink solution is immersed in an ice bath, probe-sonicate at an amplitude of 30 Hertz for 10 s (if material needs to be sterile, do this in a BSC). This step will help with further fiber dispersion but is  
5. Using a magnetic stirrer, mix the µCollaFibR™ incorporated OkaGel overnight on a hot plate at 40℃ to remove bubbles and ensure even dispersion (if material needs to be sterile, do this in a BSC).
6. The bioink solution is now ready for use in your applications.

For fiber concentrations above 5mg/mL and up to 7.5mg/mL

3. Vortex with provided metal balls at 2500-3000 RPM for 6 min.
4. While fiber solution is immersed in an ice bath, probe-sonicate at an amplitude of 30 Hertz for 10 s (if material needs to be sterile, do this in a BSC).
5. Using a magnetic stirrer, mix the µCollaFibR™ incorporated OkaGel overnight on a hot plate at 40℃ to remove bubbles and ensure even dispersion (if material needs to be sterile, do this in a BSC).
6. The bioink solution is now ready for use in your applications.